Wnt-1 and Wnt-4 regulate thymic cellularity

Eur J Immunol. 2002 Apr;32(4):967-71. doi: 10.1002/1521-4141(200204)32:4<967::AID-IMMU967>3.0.CO;2-6.

Abstract

Thymic primordium, formed by cells derived from the endoderm, the ectoderm and the neural crest-derived mesenchyme, receive fetal liver derived lymphoid precursors. Reciprocal cell-cell interactions between thymic stromal cells and lymphoid precursors are critical in the expansion and maturation of thymocytes. Transcription factor TCF-1 is critical for the expansion of thymocytes because deletion of TCF-1 results in a significant decrease in the number of thymocytes without affecting the developmental pattern. In this report we show that Wnt-1 and Wnt-4 are expressed in the thymus and the deletion of Wnt-1 or Wnt-4 result in a substantial decrease in the number of thymocytes without affecting the pattern of maturation. Wnt-1 and Wnt-4 both regulate developing thymocytes because a double deficiency results in a significantly greater decrease of immature and mature thymocytes compared to deficiency in either Wnt-1 or Wnt-4.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amphibian Proteins*
  • Animals
  • Cell Differentiation / genetics
  • Cell Division
  • Cell Survival
  • DNA, Complementary / genetics
  • DNA-Binding Proteins / physiology
  • Egg Proteins / biosynthesis
  • Egg Proteins / genetics
  • Fetal Proteins / biosynthesis
  • Fetal Proteins / genetics
  • Fetal Proteins / physiology
  • Genes, Lethal
  • Hepatocyte Nuclear Factor 1-alpha
  • Lymphocyte Count
  • Lymphoid Enhancer-Binding Factor 1
  • Mice
  • Mice, Inbred BALB C
  • Protein Biosynthesis
  • Proteins / genetics
  • Proto-Oncogene Proteins / biosynthesis
  • Proto-Oncogene Proteins / deficiency
  • Proto-Oncogene Proteins / genetics
  • Proto-Oncogene Proteins / physiology*
  • Stromal Cells / cytology
  • T Cell Transcription Factor 1
  • T-Lymphocytes / cytology*
  • Thymus Gland / cytology*
  • Thymus Gland / embryology
  • Transcription Factors / physiology
  • Wnt Proteins
  • Wnt1 Protein
  • Wnt3 Protein
  • Wnt4 Protein
  • Zebrafish Proteins*

Substances

  • Amphibian Proteins
  • DNA, Complementary
  • DNA-Binding Proteins
  • Egg Proteins
  • Fetal Proteins
  • Hepatocyte Nuclear Factor 1-alpha
  • Hnf1a protein, mouse
  • Lymphoid Enhancer-Binding Factor 1
  • Proteins
  • Proto-Oncogene Proteins
  • T Cell Transcription Factor 1
  • Transcription Factors
  • Wnt Proteins
  • Wnt1 Protein
  • Wnt1 protein, mouse
  • Wnt3 Protein
  • Wnt4 Protein
  • Wnt4 protein, mouse
  • Wnt6 protein, mouse
  • Zebrafish Proteins