Clinical impact and functional aspects of tenascin-C expression during glioma progression

Int J Cancer. 2002 Mar 20;98(3):362-9. doi: 10.1002/ijc.10233.


The extracellular matrix protein tenascin-C is expressed in processes like embryogenesis and wound healing and in neoplasia. Tenascin-C expression in gliomas has been described previously; however, the relation to clinical data remains inconsistent. Generally, analysis of tenascin-C function is difficult due to different alternatively spliced isoforms. Our studies focus on changes in tenascin-C expression in human gliomas, correlating these changes with tumor progression and elucidating the functional role of the glioma cell-specific tenascin-C isoform pool. Eighty-six glioma tissues of different World Health Organization (WHO) grades were analyzed immunohistochemically for tenascin-C expression. The influence of the specific tenascin-C isoforms produced by glioblastoma cells on proliferation and migration was examined in vitro using blocking antibodies recognizing all isoforms. In general, tenascin-C expression increased with tumor malignancy. Perivascular staining of tenascin-C around tumor-supplying blood vessels was observed in all glioblastoma tissues, whereas in WHO II and III gliomas, perivascular tenascin-C staining appeared less frequently. The appearance of perivascular tenascin-C correlated significantly with a shorter disease-free time. Analysis of proliferation and migration in the presence of blocking antibodies revealed an inhibition of proliferation by around 30% in all 3 glioblastoma cell cultures, as well as a decrease in migration of 30.6-46.7%. Thus we conclude that the endogenous pool of tenascin-C isoforms in gliomas supports both tumor cell proliferation and tumor cell migration. In addition, our data on the perivascular staining of tenascin-C in WHO II and III gliomas and its correlation with a shorter disease-free time suggest that tenascin-C may be a new and potent prognostic marker for an earlier tumor recurrence.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Monoclonal
  • Brain Neoplasms / metabolism*
  • Brain Neoplasms / physiopathology
  • Cell Division
  • Cell Movement
  • Cells, Cultured
  • Disease Progression
  • Glioblastoma / metabolism*
  • Glioblastoma / physiopathology
  • Glioma / metabolism*
  • Humans
  • Immunoenzyme Techniques
  • Neoplasm Recurrence, Local / metabolism
  • Neoplasm Staging
  • Tenascin / antagonists & inhibitors
  • Tenascin / metabolism*


  • Antibodies, Monoclonal
  • Tenascin