Tools for the Study of Genome Rearrangements in Laboratory and Industrial Yeast Strains

Yeast. 2002 Mar 30;19(5):441-8. doi: 10.1002/yea.852.

Abstract

In order to investigate the extent of genome rearrangements in laboratory and industrial yeast strains, a set of plasmids, containing ca. 300 bp fragments from highly conserved genes from S. cerevisiae, has been constructed. We chose three unique PCR products, each from a single gene, per chromosome: one from close to the centromere, and one from each chromosome end. Using these plasmids as probes to hybridize a Southern blot from a pulsed-field gel electrophoresis separation of the 16 yeast chromosomes, it is possible to identify large chromosomal rearrangements such as reciprocal translocations.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Blotting, Southern
  • Chromosome Aberrations
  • DNA Probes / chemistry
  • DNA, Fungal / chemistry
  • DNA, Fungal / genetics
  • Electrophoresis, Gel, Pulsed-Field
  • Evolution, Molecular
  • Genome, Fungal*
  • Plasmids / genetics*
  • Polymerase Chain Reaction
  • Saccharomyces cerevisiae / chemistry
  • Saccharomyces cerevisiae / genetics*

Substances

  • DNA Probes
  • DNA, Fungal