Improved silver staining protocols for high sensitivity protein identification using matrix-assisted laser desorption/ionization-time of flight analysis

Proteomics. 2001 Nov;1(11):1359-63. doi: 10.1002/1615-9861(200111)1:11<1359::AID-PROT1359>3.0.CO;2-Q.

Abstract

In proteomics it is essential to be able to detect proteins separated by gel electrophoresis at high sensitivity. Silver staining is currently the most popular method. Here we present silver staining protocols that are optimized for staining sensitivity, peptide recovery and compatibility with digestion and mass spectrometry.

MeSH terms

  • Animals
  • Carbonic Anhydrases / metabolism
  • Cattle
  • Electrophoresis, Polyacrylamide Gel / methods*
  • Proteins / chemistry*
  • Silver Staining / methods*
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*

Substances

  • Proteins
  • Carbonic Anhydrases