TREM-1, MDL-1, and DAP12 expression is associated with a mature stage of myeloid development

Mol Immunol. 2002 Mar;38(11):817-24. doi: 10.1016/s0161-5890(02)00004-4.


The triggering receptor expressed on myeloid cells (TREM-1) and the myeloid DAP12-associating lectin (MDL-1) are two recently identified receptors which associate non-covalently with DAP12 to form receptor complexes involved in monocytic activation and inflammatory response. In this study, we investigated whether the expression of TREM-1, MDL-1, and DAP12 correlated with myelomonocytic differentiation. Northern and RT-PCR revealed a strong expression of TREM-1, MDL-1, and DAP12 in peripheral blood-derived CD14(+) mature monocytes in contrast to undifferentiated bone marrow CD34(+) stem cells, and in the differentiated versus undifferentiated U937 cells. TREM-1 and MDL-1 RNA expression was also more elevated in adult than fetal tissues and in normal than malignant cells. These findings suggest that the TREM-1/DAP12 and MDL-1/DAP12 signaling pathways are features of mature differentiated myelomonocytic cells. In addition, expression of an alternative mRNA TREM-1 splice variant (TREM-1sv) was detected that might translate into a soluble receptor with potential as a regulator of myeloid activation.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adaptor Proteins, Signal Transducing
  • Amino Acid Sequence
  • Base Sequence
  • Cell Differentiation
  • Hematopoietic Stem Cells / metabolism*
  • Humans
  • Lectins, C-Type*
  • Leukemia / metabolism
  • Membrane Glycoproteins*
  • Membrane Proteins
  • Molecular Sequence Data
  • Monocytes / metabolism*
  • RNA, Messenger / analysis
  • Receptors, Cell Surface / genetics*
  • Receptors, Immunologic / genetics*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Triggering Receptor Expressed on Myeloid Cells-1


  • Adaptor Proteins, Signal Transducing
  • CLEC5A protein, human
  • Lectins, C-Type
  • Membrane Glycoproteins
  • Membrane Proteins
  • RNA, Messenger
  • Receptors, Cell Surface
  • Receptors, Immunologic
  • TREM1 protein, human
  • TREM2 protein, human
  • TYROBP protein, human
  • Triggering Receptor Expressed on Myeloid Cells-1

Associated data

  • GENBANK/AI391640
  • GENBANK/AI394041
  • GENBANK/AI394092