Organization of the lipoprotein lipase gene of red sea bream Pagrus major

Comp Biochem Physiol B Biochem Mol Biol. 2002 Apr;131(4):775-85. doi: 10.1016/s1096-4959(02)00035-0.


Lipoprotein lipase (LPL) is a key enzyme of lipid deposition and metabolism. To investigate the mechanism of lipid deposition in fish, as a first step, we have characterized the LPL gene of a marine teleost red sea bream Pagrus major by cDNA and genomic structure analysis. The red sea bream LPL gene encodes 511 amino acids and spans approximately 6.3 kb of the genome. The coding region is organized into ten exons and nine introns. In comparison with the LPL of other animals, the deduced amino acid sequence shows a high degree of similarity with a conservation of functional domains, e.g. catalytic triad, N-glycosylation sites, lipid and heparin binding regions. The 1.1 kb of 5' flanking region contains two CCAAT, sequences homologous to Oct-I site and response elements for hormones including glucocorticoid, insulin and thyroid hormone. The results of the present study will facilitate further study of the function and regulation of the LPL in non-mammalian vertebrates.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Binding Sites
  • Chromosome Mapping
  • Conserved Sequence
  • DNA, Complementary / metabolism
  • Exons
  • Introns
  • Lipoprotein Lipase / genetics*
  • Lipoprotein Lipase / metabolism*
  • Models, Genetic
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • RNA / metabolism
  • Sea Bream / metabolism*
  • Sequence Homology, Nucleic Acid


  • DNA, Complementary
  • RNA
  • Lipoprotein Lipase