Transport of insulin-like growth factor-I across endothelial cell monolayers and its binding to the subendothelial matrix

Exp Clin Endocrinol Diabetes. 2002 Apr;110(2):67-73. doi: 10.1055/s-2002-23488.


Cultured human umbilical vein endothelial cells (HUVEC) were used as a model to study transendothelial IGF-I transport, and its deposition into the extracellular matrix (ECM). Specific binding of (125)I-IGF-I to HUVEC monolayers was demonstrated, which was inhibited by aIR-3, a specific antibody directed against the IGF-I receptor. ECM-associated (125)I-IGF-I was approximately 10% of cell-bound IGF-I at 22 degrees C, and increased 4.5-fold at 37 degrees C, indicating that endothelial metabolism is required for the transport. However, neither monensin and cytochalasin B, both of which block endocytosis, nor aIR-3 did inhibit transport of (125)I-IGF-I into the ECM. In order to characterize IGF-I binding to the subendothelial ECM, HUVEC were removed nonenzymatically by treatment with Triton X-100 and ammonia. Specific, saturable binding of (125)I-IGF-I to the isolated ECM was observed, which was protease-sensitive. Antibodies directed against vitronectin inhibited IGF-I binding to the matrix by 35%, while antibodies directed against other ECM proteins had no significant influence on IGF-I binding. Using radioimmunoassays the IGF binding protein-2 was detected in the ECM, while IGFBP-1 and IGFBP-3 were below the detection limits. In order to evaluate functional aspects of IGF-I binding to the matrix, HUVEC were incubated under serum-free conditions in the absence and presence of IGF-I. Under serum-free conditions 48% of cells rounded up and started to detach after 2 hours incubation, while only 23% of the cells started to detach in the presence of IGF-I. These data indicate that IGF-I is transported via a paracellular route across endothelial cells, and becomes bound to the subendothelial ECM. Vitronection seems to be involved in binding of IGF-I to the ECM. ECM-associated IGF-I might play a role in endothelial cell survival and stability.

MeSH terms

  • Ammonia / pharmacology
  • Antibodies / pharmacology
  • Biological Transport
  • Cell Adhesion
  • Cells, Cultured
  • Culture Media, Serum-Free
  • Endothelium, Vascular / metabolism*
  • Extracellular Matrix / metabolism*
  • Humans
  • Insulin-Like Growth Factor Binding Protein 2 / analysis
  • Insulin-Like Growth Factor I / metabolism*
  • Insulin-Like Growth Factor I / pharmacology
  • Iodine Radioisotopes
  • Octoxynol / pharmacology
  • Umbilical Veins
  • Vitronectin / immunology
  • Vitronectin / physiology


  • Antibodies
  • Culture Media, Serum-Free
  • Insulin-Like Growth Factor Binding Protein 2
  • Iodine Radioisotopes
  • Vitronectin
  • Insulin-Like Growth Factor I
  • Ammonia
  • Octoxynol