Quantitation of gamma-trace in human biological fluids: indications for production in the central nervous system

Scand J Clin Lab Invest. 1979 Nov;39(7):619-26. doi: 10.3109/00365517909108866.


Gamma-Trace was purified in large amounts from urine and used for the production of a specific rabbit antiserum. An enzyme immunoassay for quantitation of gamma-trace was developed using the pure protein as a primary standard. Its sensitivity was approximately 30 microgram/l. An enzyme amplified single radial immunodiffusion was developed as well. Its sensitivity was approximately 0.3 mg/l. These assays allowed quantitation of gamma-trace in normal human biological fluids. The following results were obtained (mean +/- SD): cerebrospinal fluid: 5.8 +/- 2.2 mg/l, plasma: 1.1 +/- 0.42 mg/l, saliva: 1.8 +/- 0.88 mg/l and urine: 0.095 +/- 0.057 mg/l. Plasma samples from patients with advanced renal failure revealed gamma-trace values up to 13 times the normal mean plasma value. The results indicate a production of gamma-trace in the central nervous system and that the protein is primarily catabolized by the kidney.

MeSH terms

  • Cerebrospinal Fluid Proteins / analysis*
  • Cerebrospinal Fluid Proteins / blood
  • Cerebrospinal Fluid Proteins / urine
  • Globulins / analysis*
  • Globulins / cerebrospinal fluid
  • Globulins / urine
  • Humans
  • Immunodiffusion
  • Immunoenzyme Techniques*
  • Saliva / analysis


  • Cerebrospinal Fluid Proteins
  • Globulins