Salinibacter ruber gen. nov., sp. nov., a novel, extremely halophilic member of the Bacteria from saltern crystallizer ponds

Int J Syst Evol Microbiol. 2002 Mar;52(Pt 2):485-91. doi: 10.1099/00207713-52-2-485.


Five brightly red-pigmented, motile, rod-shaped, extremely halophilic bacteria were isolated from saltern crystallizer ponds in Alicante (two strains) and Mallorca (three strains), Spain. They grew optimally at salt concentrations between 20 and 30% and did not grow below 15% salts. Thus, these isolates are among the most halophilic organisms known within the domain Bacteria. The temperature optimum was 37-47 degrees C. A single, yet to be identified pigment was present, with an absorption maximum at 482 nm and a shoulder at 506-510 nm. The G+C content of the DNA was 66.3-67.7 mol% and, together, they formed a homogeneous genomic group with DNA-DNA similarities above 70%. The 16S rRNA gene sequences were almost identical to sequences recovered earlier from the saltern biomass by amplification of bacterial small-subunit rRNA genes from DNA extracted from the environment. This phylotype, earlier described as 'Candidatus Salinibacter', was shown by fluorescence in situ hybridization to contribute between 5 and 25% of the prokaryote community of the saltern crystallizers. We have therefore succeeded in isolating a bacterium from the natural environment that, although being a major component of the community, was previously known by its phylotype only. Isolation of the organism now allows formal description of a novel genus and species, for which we propose the name Salinibacter ruber gen. nov., sp. nov. The type strain is strain M31T (= DSM 13855T = CECT 5946T).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacteria / classification*
  • Bacteria / genetics
  • Bacteria / isolation & purification
  • Bacterial Physiological Phenomena
  • Base Composition
  • DNA, Bacterial / chemistry
  • Fresh Water
  • Molecular Sequence Data
  • Phylogeny
  • Pigments, Biological
  • RNA, Ribosomal / chemistry
  • RNA, Ribosomal, 16S / chemistry
  • Sequence Homology, Nucleic Acid
  • Sodium Chloride
  • Spain
  • Species Specificity
  • Temperature


  • DNA, Bacterial
  • Pigments, Biological
  • RNA, Ribosomal
  • RNA, Ribosomal, 16S
  • Sodium Chloride

Associated data

  • GENBANK/AF323499
  • GENBANK/AF323500
  • GENBANK/AF323501
  • GENBANK/AF323502