Accuracy and precision of intraocular injection volume

Am J Ophthalmol. 2002 Apr;133(4):564-6. doi: 10.1016/s0002-9394(01)01408-8.


Purpose: To assess the accuracy and precision of methods in clinical use for intraocular drug injection.

Design: Experimental laboratory investigation.

Methods: A total of 800 injections were performed using 20 different 1-ml syringes at intended volumes of 0.10 ml and 0.05 ml. The volume of each injection was calculated from mass of water delivered onto an analytic balance.

Results: The mean measured volume delivered was less than the intended volume for every syringe (P <.001 for each syringe at each volume). A total of 7% and 22% of injections differed by 20% or more from the intended volumes of 0.10 ml and 0.05 ml, respectively. Overdelivery of volume was uncommon.

Conclusion: Clinically relevant materials and methods used for intraocular injection of drugs systematically underdelivered the intended volume.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Injections / standards*
  • Ophthalmology / standards
  • Reference Standards
  • Reproducibility of Results
  • Syringes / standards*
  • Weights and Measures / standards*