IL-15 regulation in human endometrial stromal cells

J Clin Endocrinol Metab. 2002 Apr;87(4):1898-901. doi: 10.1210/jcem.87.4.8539.


A greater knowledge of IL-5 regulation within human endometrium is important in understanding key reproductive events and uterine Natural Killer cell function. In the present study, expression of IL-15 mRNA was shown to be up-regulated by both PGE(2) and IFN-gamma in cultures of human endometrial stromal cells (ESC). Release of IL-15 protein was also shown to be under the control of PGE(2) and IFN-gamma using an enzyme-linked immunosorbent assay for IL-15. In addition, 8-Bromo cAMP was able to increase IL-15 release from ESCs (P < 0.005) implying the actions of PGE(2) may be via this second messenger. Addition of a progestin appeared to enhance these effects. Real-time quantitative PCR has demonstrated an up-regulation in IL-15 mRNA expression in the late secretory phase of the menstrual cycle (P < 0.005) and a progressive rise in IFN-gamma expression throughout the secretory phase and into first trimester decidua. These results suggest that IL-15 regulation in the human endometrium is complex and that hormonal control may be indirect.

MeSH terms

  • 8-Bromo Cyclic Adenosine Monophosphate / pharmacology
  • Adult
  • Cells, Cultured
  • Dinoprostone / pharmacology
  • Drug Combinations
  • Endometrium / drug effects
  • Endometrium / metabolism*
  • Female
  • Humans
  • Interferon-gamma / metabolism
  • Interferon-gamma / pharmacology
  • Interleukin-15 / genetics
  • Interleukin-15 / metabolism*
  • Medroxyprogesterone Acetate / pharmacology
  • Menstrual Cycle / physiology
  • Progesterone Congeners
  • RNA, Messenger / metabolism
  • Reverse Transcriptase Polymerase Chain Reaction
  • Stromal Cells / drug effects
  • Stromal Cells / metabolism*
  • Up-Regulation


  • Drug Combinations
  • Interleukin-15
  • Progesterone Congeners
  • RNA, Messenger
  • 8-Bromo Cyclic Adenosine Monophosphate
  • Interferon-gamma
  • Medroxyprogesterone Acetate
  • Dinoprostone