Two new steroids, 3 beta-vanilloyloxy-stigmast-5-ene-7-one, and stigmast-5-ene-3 beta,7 beta,15 alpha-triol were isolated from the roots of Adenophora stenanthina subsp. xifengensis along with four known compounds, stigmast-5-ene-3 beta-ol-7-one, stigmast-5-ene-3 beta,7 beta-diol, syringin, sinapyl alcohol 1, 3'-di-O-beta-D-glucopyranoside. Their structures were elucidated on the basis of spectral methods.