Synthesis of two neuromedin U (NMU) analogues and their comparative effect of reducing food intake in rats

Prep Biochem Biotechnol. 2002 Feb;32(1):79-86. doi: 10.1081/PB-120013163.

Abstract

To examine the roles of aromatic rings, Tyr residues at positions 1 and 5 and Phe residues at positions 16, 17, and 19 of rat neuromedin U-23 (NMU-23) (Tyr-Lys-Val-Asn-Glu-Tyr-Gln-Gly-Pro-Val-Ala-Pro-Ser-Gly-Gly-Phe-Phe-Leu-Phe-Arg-Pro-Arg-Asn-NH2) for reducing food intake activity in male Wistar rats, two NMU-23 analogues, [Phe(4F)16,17,19] NMU-23 and [Tyr(Me)1,6]NMU-23, were synthesized by Fmoc strategy of manual solid-phase method. The synthetic NMU-23 showed reducing effect on food intake in rats. [Phe(4F)16,17,19]NMU-23 exhibited higher reducing food in take effect than that of NMU-23. On the contrary, [Tyr(- Me)1,6]NMU-23 showed no reducing effect on food intake in rats than that of NMU-23.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Chromatography, High Pressure Liquid
  • Chromatography, Thin Layer
  • Feeding Behavior / drug effects*
  • Injections, Intraventricular
  • Male
  • Molecular Sequence Data
  • Neuropeptides / administration & dosage
  • Neuropeptides / chemical synthesis*
  • Neuropeptides / isolation & purification
  • Neuropeptides / pharmacology*
  • Phenylalanine / metabolism
  • Protein Engineering
  • Rats
  • Rats, Wistar
  • Spectrometry, Mass, Fast Atom Bombardment
  • Structure-Activity Relationship
  • Tyrosine / metabolism

Substances

  • Neuropeptides
  • neuromedin U
  • Tyrosine
  • Phenylalanine