Diversity and phylogenetic analysis of bacteria in the mucosa of chicken ceca and comparison with bacteria in the cecal lumen

FEMS Microbiol Lett. 2002 Feb 19;208(1):1-7. doi: 10.1111/j.1574-6968.2002.tb11051.x.

Abstract

We reported the first attempt to describe mucosa-associated bacterial populations in the chicken ceca by molecular analysis of 16S rRNA genes. Bacteria in the mucosa were highly diverse but mainly Gram-positive with low G+C. Fusobacterium prausnitzii and butyrate-producing bacteria comprised the largest groups among 116 cloned sequences. Twenty five percent of the clones had less than 95% homology to database sequences. Many sequences were related to those of uncultured bacteria identified in human feces or the bovine rumen. Terminal restriction fragment length polymorphism (T-RFLP) analysis revealed some differences between bacterial populations present in the mucosa and lumen of ceca. Greater resolution of bacterial population was obtained using a culture-independent approach rather than a culture-based approach.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Bacteria / classification*
  • Bacteria / genetics
  • Bacteria / isolation & purification
  • Cecum / microbiology*
  • Chickens / microbiology*
  • Culture Media
  • Ecosystem
  • Genes, rRNA
  • Genetic Variation
  • Intestinal Mucosa / microbiology*
  • Molecular Sequence Data
  • Phylogeny*
  • Polymorphism, Restriction Fragment Length
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA

Substances

  • Culture Media
  • RNA, Ribosomal, 16S

Associated data

  • GENBANK/AF429354
  • GENBANK/AF429355
  • GENBANK/AF429356
  • GENBANK/AF429357
  • GENBANK/AF429358
  • GENBANK/AF429359
  • GENBANK/AF429360
  • GENBANK/AF429361
  • GENBANK/AF429362
  • GENBANK/AF429363
  • GENBANK/AF429364
  • GENBANK/AF429365
  • GENBANK/AF429366
  • GENBANK/AF429367
  • GENBANK/AF429368
  • GENBANK/AF429369
  • GENBANK/AF429370
  • GENBANK/AF429371
  • GENBANK/AF429372
  • GENBANK/AF429373
  • GENBANK/AF429374
  • GENBANK/AF429375
  • GENBANK/AF429376
  • GENBANK/AF429377
  • GENBANK/AF429378
  • GENBANK/AF429379
  • GENBANK/AF429380
  • GENBANK/AF429381
  • GENBANK/AF429382