Enzymatic synthesis of labeled DNA by PCR using new fluorescent thymidine nucleotide analogue and superthermophilic KOD dash DNA polymerase

Bioorg Med Chem Lett. 2002 Apr 22;12(8):1167-70. doi: 10.1016/s0960-894x(02)00111-7.


Triphosphate of a new fluorescent labeled thymidine analogue was incorporated as a substrate for PCR using KOD Dash DNA polymerase forming the corresponding fluorescent labeled DNA which is useful for a DNA probe.

MeSH terms

  • Base Sequence
  • DNA / chemical synthesis*
  • DNA Probes
  • DNA-Directed DNA Polymerase / metabolism*
  • Fluorescent Dyes / metabolism*
  • Nucleotides / metabolism*
  • Polymerase Chain Reaction
  • Thymidine / metabolism*


  • DNA Probes
  • Fluorescent Dyes
  • Nucleotides
  • DNA
  • DNA-Directed DNA Polymerase
  • Thymidine