Vitamin C ameliorates fluoride-induced embryotoxicity in pregnant rats

Hum Exp Toxicol. 2001 Dec;20(12):619-23. doi: 10.1191/096032701718890559.


Oral administration of sodium fluoride (40 mg/kg body weight) from day 6 to 19 of gestation caused, as compared to control, significant reductions in body weight, feed consumption, absolute uterine weight and number of implantations. Significantly higher incidence of skeletal (wavy ribs, 14th rib, <6 sternal centre, dumbell-shaped second and fifth sternebrae, incomplete ossification of skull and thickening of tibia) and visceral (subcutaneous haemorrhage) abnormalities were also observed in NaF-treated dams than that of control. Oral administration of vitamin C (50 mg/kg body weight) and vitamin E (2 mg/0.2 ml olive oil/animal/day) from day 6 to 19 of gestation along with NaF significantly ameliorates NaF-induced reductions in body weight, feed consumption, absolute uterine weight (only with vitamin E treatment) and number of implantations. As compared with NaF-treated alone, the total percentage of skeletal and visceral abnormalities were significantly lowered in fluoride plus vitamin C-treated animals. Vitamin E was less effective. These findings suggest that vitamin C significantly reduced the severity and incidence of fluoride-induced embryotoxicity in rats.

MeSH terms

  • Abnormalities, Drug-Induced / prevention & control*
  • Administration, Oral
  • Animals
  • Ascorbic Acid / administration & dosage
  • Ascorbic Acid / therapeutic use*
  • Body Weight / drug effects
  • Bone and Bones / abnormalities
  • Bone and Bones / drug effects
  • Eating / drug effects
  • Embryo Implantation / drug effects
  • Embryo, Mammalian / drug effects*
  • Embryo, Mammalian / pathology
  • Female
  • Organ Size / drug effects
  • Pregnancy
  • Rats
  • Rats, Wistar
  • Sodium Fluoride / administration & dosage
  • Sodium Fluoride / toxicity*
  • Teratogens / toxicity*
  • Uterus / drug effects
  • Vitamin E / administration & dosage
  • Vitamin E / therapeutic use


  • Teratogens
  • Vitamin E
  • Sodium Fluoride
  • Ascorbic Acid