PCR-RFLP analysis of Giardia intestinalis using a Giardia-specific gene, GLORF-C4

Parasite. 2002 Mar;9(1):65-70. doi: 10.1051/parasite/200209165.

Abstract

A cDNA clone encoding GLORF-C4 was isolated from the WB strain, an assemblage A Giardia intestinalis. Interestingly, GLORF-C4 has been previously reported as an assemblage B-specific gene. Using two primers based on GLORF-C4 of the GS strain, a prototype assemblage B, GLORF-C4 gene was amplified from all the groups of G. intestinalis, and applied to detect the presence of cysts of G. intestinalis from faecal samples of cyst-passers. RFLP analysis of this PCR product successfully classified G. intestinalis into two distinct groups, assemblages A and B.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • China
  • DNA Primers
  • Feces / parasitology
  • Genes, Protozoan*
  • Giardia lamblia / classification
  • Giardia lamblia / genetics*
  • Giardiasis / parasitology
  • Humans
  • Intestinal Diseases, Parasitic / parasitology
  • Korea
  • Laos
  • Molecular Sequence Data
  • Polymerase Chain Reaction*
  • Polymorphism, Restriction Fragment Length*
  • Protozoan Proteins / genetics*
  • Sequence Alignment
  • Species Specificity

Substances

  • DNA Primers
  • GLORF-C4 protein, Giardia lamblia
  • Protozoan Proteins