Characterization of two TCR transgenic mouse lines specific for herpes simplex virus

Immunol Cell Biol. 2002 Apr;80(2):156-63. doi: 10.1046/j.1440-1711.2002.01071.x.


To better understand the T cell-mediated processes involved in the immune response to herpes simplex virus type 1 (HSV-1)infection, two HSV-specific T cell receptor (TCR) transgenic mouse lines were produced. These mice (gBT-I.1 and gBT-I.3) are MHC class I-restricted and specific for the immunodominant peptide from HSV glycoprotein B (gB), gB498-505. Although derived from the same clone, the mice differ in the chromosomal location of the TCR transgenes and show marked differences in TCR alpha/beta expression on both CD4+ and CD8+ cells in the thymus. Despite this, peripheral CD8+ Tcells from both mice express equally high levels of the transgenic TCR and bind the KbgB498-505 tetramer to the same degree. In concordance with this, both were shown to respond equally well in vitro upon stimulation with the gB498-505 peptide or HSV-infected cells. These data show that selection of broadly equivalent peripheral T-cell subsets can occur in the presence of distinctly different thymic T-cell subsets.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cells, Cultured
  • Clone Cells
  • Cytotoxicity, Immunologic
  • Flow Cytometry
  • Genes, MHC Class I
  • Mice
  • Mice, Inbred C57BL
  • Mice, Transgenic / immunology*
  • Receptors, Antigen, T-Cell / chemistry
  • Receptors, Antigen, T-Cell / genetics*
  • Simplexvirus / immunology*
  • T-Lymphocytes / immunology
  • T-Lymphocytes, Cytotoxic / immunology*
  • Viral Envelope Proteins / genetics
  • Viral Envelope Proteins / immunology*


  • Receptors, Antigen, T-Cell
  • Viral Envelope Proteins
  • glycoprotein B, Simplexvirus