Novel nuclear-encoded proteins interacting with a plastid sigma factor, Sig1, in Arabidopsis thaliana

FEBS Lett. 2002 Mar 13;514(2-3):300-4. doi: 10.1016/s0014-5793(02)02388-8.

Abstract

Sigma factor binding proteins are involved in modifying the promoter preferences of the RNA polymerase in bacteria. We found the nuclear encoded protein (SibI) that is transported into chloroplasts and interacts specifically with the region 4 of Sig1 in Arabidopsis. SibI and its homologue, T3K9.5 are novel proteins, which are not homologous to any protein of known function. The expression of sibI was tissue specific, light dependent, and developmentally timed. We suggest the transcriptional regulation by sigma factor binding proteins to function in the plastids of higher plant.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis / metabolism*
  • Arabidopsis Proteins / genetics
  • Arabidopsis Proteins / metabolism*
  • Base Sequence
  • Blotting, Northern
  • Chloroplasts / metabolism
  • DNA, Complementary / genetics
  • DNA, Complementary / isolation & purification
  • Gene Expression Regulation, Developmental / radiation effects
  • Gene Expression Regulation, Plant / radiation effects
  • Green Fluorescent Proteins
  • Light
  • Luminescent Proteins / genetics
  • Molecular Sequence Data
  • Nuclear Proteins / genetics
  • Nuclear Proteins / metabolism*
  • Plant Proteins / genetics
  • Plant Proteins / metabolism
  • Protein Binding / physiology
  • Protein Transport / physiology
  • RNA, Messenger / metabolism
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Sigma Factor / genetics
  • Sigma Factor / metabolism*
  • Substrate Specificity

Substances

  • Arabidopsis Proteins
  • DNA, Complementary
  • Luminescent Proteins
  • Nuclear Proteins
  • Plant Proteins
  • RNA, Messenger
  • Recombinant Fusion Proteins
  • SIB1 protein, Arabidopsis
  • Sigma Factor
  • sigma factor Sig1
  • Green Fluorescent Proteins

Associated data

  • GENBANK/AF224762