A filtration, incubation and staining reactor including a new protocol for FISH

J Microbiol Methods. 2002 Jun;50(1):97-100. doi: 10.1016/s0167-7012(02)00014-3.

Abstract

A newly developed device for performing fluorescence in situ hybridization (FISH) is described. An adapted procedure was compared with two typical FISH protocols. Tests were performed with Pseudomonas cells and the gene probe EUB338. With the novel procedure, we obtained a better recovery of cells and less variability in results.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Technical Report

MeSH terms

  • Artifacts
  • Filtration / methods*
  • In Situ Hybridization, Fluorescence / instrumentation*
  • In Situ Hybridization, Fluorescence / methods*
  • Molecular Probes
  • Pseudomonas / genetics
  • RNA, Bacterial / analysis*
  • RNA, Ribosomal, 16S / analysis*
  • Reproducibility of Results
  • Staining and Labeling / methods

Substances

  • Molecular Probes
  • RNA, Bacterial
  • RNA, Ribosomal, 16S