Should gaps be included in chromosomal aberration analysis? Evidence based on the comet assay

Mutat Res. 2002 Apr 26;516(1-2):57-61. doi: 10.1016/s1383-5718(02)00021-9.

Abstract

This study evaluated DNA damage in human lymphocytes due to occupational exposure to low levels of ionizing radiation using two assays: the comet assay and chromosomal aberration (CA) analysis including and excluding gaps. The results obtained reveal a higher correlation between both methods when chromatid and chromosome gaps were included in the correlation analysis (r=0.78 versus r=0.50). This increased correlation support the hypothesis that the gaps constitute a type of chromosome aberration, and suggest that these events should be scored in this type of analysis.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Chromosome Aberrations / radiation effects*
  • Comet Assay / methods*
  • DNA Damage / radiation effects*
  • Dose-Response Relationship, Radiation
  • Environmental Monitoring
  • Female
  • Health Personnel
  • Humans
  • Lymphocytes / radiation effects
  • Male
  • Middle Aged
  • Occupational Exposure / analysis*
  • Radiation Injuries / diagnosis*
  • Radiation Injuries / genetics
  • Radiation Monitoring / methods
  • Radiation, Ionizing*
  • Radiology