Specificity of autoantibodies to SS-A/Ro on a transfected and overexpressed human 60 kDa Ro autoantigen substrate

J Clin Lab Anal. 2002;16(2):103-8. doi: 10.1002/jcla.10026.

Abstract

The objective of this study was to analyze apparently discrepant results that arose during the use of an indirect immunofluorescence (IIF) assay using transfected HEp-2 cells to detect anti-SS-A/Ro autoantibodies in human sera. Fourteen sera that had SS-A/Ro antibodies as detected on this commercial substrate, but did not have antibodies to SS-A/Ro as determined by double immunodiffusion (ID) or enzyme-linked immunosorbent assay (ELISA), were studied by immunoprecipitation (IP) of radiolabeled cell extracts and full-length recombinant SS-A/Ro. A multi-antigen strip immunoblotting (IB) assay containing both the 52- and 60-kDa antigens was included in the analysis. We confirmed that 12 of 14 of the sera under study had antibodies to SS-A/Ro protein antigens as determined by at least one other immunoassay. One serum had antibodies to hyRNA but no detectable reactivity with the 52- or 60-kDa antigens. One serum remained negative in all assays for SS-A/Ro autoantibodies.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Autoantibodies / blood*
  • Autoantigens / immunology*
  • Biomarkers / blood
  • Enzyme-Linked Immunosorbent Assay*
  • Fluorescent Antibody Technique, Indirect*
  • Humans
  • RNA, Small Cytoplasmic*
  • Ribonucleoproteins / immunology*
  • Sensitivity and Specificity
  • Sjogren's Syndrome / diagnosis*
  • Sjogren's Syndrome / immunology
  • Transfection*

Substances

  • Autoantibodies
  • Autoantigens
  • Biomarkers
  • RNA, Small Cytoplasmic
  • Ribonucleoproteins
  • SS-A antigen
  • TROVE2 protein, human