Abstract
The oxidation of DNA and RNA provides a facile approach for investigating the interaction of nucleic acids with proteins and oligonucleotides. In this article, we have outlined our understanding of the mechanism of DNA scission by 1,10-phenanthroline-copper(I) in the presence of hydrogen peroxide. We also discuss results obtained by using 1,10-phenanthroline-oligonucleotide conjugates in probing the size of the transcriptionally active open complex. Finally, we outline an effective method for converting DNA-binding proteins into site-specific modification agents by using 1,10-phenanthroline-copper(I).
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Base Sequence
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DNA / chemistry
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DNA / metabolism*
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DNA-Binding Proteins / antagonists & inhibitors
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DNA-Binding Proteins / metabolism
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Deoxyribonucleases / chemical synthesis*
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Deoxyribonucleases / metabolism*
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Enzyme Inhibitors / pharmacology
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Hydrogen Peroxide / pharmacology
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Macromolecular Substances
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Models, Molecular
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Molecular Conformation
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Oxidation-Reduction
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Phenanthrolines / pharmacology
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Phosphates / pharmacology
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Substrate Specificity
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Transcription, Genetic / drug effects
Substances
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DNA-Binding Proteins
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Enzyme Inhibitors
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Macromolecular Substances
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Phenanthrolines
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Phosphates
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phosphorodithioic acid
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DNA
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Hydrogen Peroxide
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Deoxyribonucleases
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1,10-phenanthroline