Artificial nucleases

Chembiochem. 2001 Oct 1;2(10):735-40. doi: 10.1002/1439-7633(20011001)2:10<735::aid-cbic735>3.0.co;2-#.

Abstract

The oxidation of DNA and RNA provides a facile approach for investigating the interaction of nucleic acids with proteins and oligonucleotides. In this article, we have outlined our understanding of the mechanism of DNA scission by 1,10-phenanthroline-copper(I) in the presence of hydrogen peroxide. We also discuss results obtained by using 1,10-phenanthroline-oligonucleotide conjugates in probing the size of the transcriptionally active open complex. Finally, we outline an effective method for converting DNA-binding proteins into site-specific modification agents by using 1,10-phenanthroline-copper(I).

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Base Sequence
  • DNA / chemistry
  • DNA / metabolism*
  • DNA-Binding Proteins / antagonists & inhibitors
  • DNA-Binding Proteins / metabolism
  • Deoxyribonucleases / chemical synthesis*
  • Deoxyribonucleases / metabolism*
  • Enzyme Inhibitors / pharmacology
  • Hydrogen Peroxide / pharmacology
  • Macromolecular Substances
  • Models, Molecular
  • Molecular Conformation
  • Oxidation-Reduction
  • Phenanthrolines / pharmacology
  • Phosphates / pharmacology
  • Substrate Specificity
  • Transcription, Genetic / drug effects

Substances

  • DNA-Binding Proteins
  • Enzyme Inhibitors
  • Macromolecular Substances
  • Phenanthrolines
  • Phosphates
  • phosphorodithioic acid
  • DNA
  • Hydrogen Peroxide
  • Deoxyribonucleases
  • 1,10-phenanthroline