Identification of TH1 as an interaction partner of A-Raf kinase

Mol Cell Biochem. 2002 Feb;231(1-2):69-74. doi: 10.1023/a:1014437024129.


A-Raf is an important intermediate of the growth factor Ras-MAP kinase pathway. In a two-hybrid screen of human fetal liver cDNA library, TH1 was detected as a new interaction partner of A-Raf. TH1 is a highly conserved and widely expressed protein, which was recently cloned by Bonthron DT group. The binding between A-Raf and TH1 was specific, as no binding between TH1 and B-Raf or c-Raf was observed, and the amino-terminal 162 amino acids in the A-Raf regulatory domain were found to be sufficient for this interaction. This specific interaction may have played a critical role in the activation of A-Raf.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Blotting, Western
  • Cell Line
  • Humans
  • Liver / metabolism
  • Phosphorylation
  • Protein Binding
  • Proto-Oncogene Proteins A-raf
  • Proto-Oncogene Proteins c-raf / genetics
  • Proto-Oncogene Proteins c-raf / metabolism*
  • Substrate Specificity
  • Two-Hybrid System Techniques


  • Proto-Oncogene Proteins A-raf
  • Proto-Oncogene Proteins c-raf