Inhibition by long-term fermented miso of induction of gastric tumors by N-methyl-N'-nitro-N-nitrosoguanidine in CD (SD) rats

Oncol Rep. May-Jun 2002;9(3):613-6.


The present study was designed to investigate the effects of fermented miso in the diet on the induction of gastric tumors by N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) in male CD (SD) rats. A total of 120 animals, 6 weeks of age, were divided into 6 groups and given MNNG (100 ppm) in the drinking water for 16 weeks. Starting 1 week before the carcinogen treatment the rats were fed a normal control MF solid diet, or the same diet containing 10% long-term fermented, medium- or short-term fermented miso, or 1% NaCl until the end of the MNNG exposure period. They were then maintained on the MF control diet and normal tap water until the autopsy time point at 52 weeks. The long-term fermented miso significantly reduced the size of the gastric tumors as compared with the other groups. The present results thus indicate that dietary supplementation with long-term fermented miso could act as a chemopreventive agent for gastric carcinogenesis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Avena / metabolism
  • Body Weight / drug effects
  • Carcinogens*
  • Fermentation
  • Male
  • Methylnitronitrosoguanidine*
  • Organ Size / drug effects
  • Oryza / metabolism
  • Plant Extracts / therapeutic use*
  • Rats
  • Soybeans / metabolism
  • Stomach Neoplasms / chemically induced*
  • Stomach Neoplasms / prevention & control*
  • Time Factors
  • Triticum / metabolism


  • Carcinogens
  • Plant Extracts
  • Methylnitronitrosoguanidine