Selective induction of apoptosis by ar-turmerone isolated from turmeric (Curcuma longa L) in two human leukemia cell lines, but not in human stomach cancer cell line

Int J Mol Med. 2002 May;9(5):481-4.

Abstract

We have investigated the effects of ar-turmerone isolated from turmeric (Curcuma longa L) on DNA of human leukemia cell lines, Molt 4B, HL-60 and stomach cancer KATO III cells. It was found that selective induction of apoptosis by ar-turmerone was observed in human leukemia Molt 4B and HL-60 cells, but not in human stomach cancer KATO III cells. Morphological changes showing apoptotic bodies were observed in the human HL-60 and Molt 4B cells treated with ar-turmerone. The fragmentation of DNA by ar-turmerone to oligonucleosomal-sized fragments that is a characteristic of apoptosis was observed to be concentration- and time-dependent in Molt 4B and HL-60 cells, but not in KATO III cells. The data of the present study show that the suppression by ar-turmerone of growth of these leukemia cell lines results from the induction of apoptosis by this compound.

MeSH terms

  • Apoptosis / drug effects*
  • Cell Division / drug effects
  • Chromatography, High Pressure Liquid
  • Curcuma / chemistry*
  • DNA Fragmentation / drug effects
  • HL-60 Cells
  • Humans
  • Ketones / chemistry
  • Ketones / isolation & purification*
  • Ketones / pharmacology*
  • Ketones / therapeutic use
  • Leukemia / drug therapy
  • Leukemia / pathology*
  • Lymphocytes / cytology
  • Lymphocytes / drug effects
  • Magnetic Resonance Spectroscopy
  • Sesquiterpenes
  • Stomach Neoplasms / pathology*
  • Toluene / analogs & derivatives*
  • Toluene / chemistry
  • Toluene / isolation & purification*
  • Toluene / pharmacology*
  • Toluene / therapeutic use
  • Tumor Cells, Cultured

Substances

  • Ketones
  • Sesquiterpenes
  • ar-turmerone
  • Toluene