Isolation and Characterization of a New Succinic Acid-Producing Bacterium, Mannheimia succiniciproducensMBEL55E, From Bovine Rumen

Appl Microbiol Biotechnol. 2002 Apr;58(5):663-8. doi: 10.1007/s00253-002-0935-6. Epub 2002 Feb 8.

Abstract

A novel succinic acid-producing bacterium was isolated from bovine rumen. The bacterium is a non-motile, non-spore-forming, mesophilic and capnophilic gram-negative rod or coccobacillus. Phylogenetic analysis based on the 16S rRNA sequence and physiological analysis indicated that the strain belongs to the recently reclassified genus Mannheimia as a novel species, and has been named Mannheimia succiniciproducens MBEL55E. Under 100% CO(2) conditions, it grows well in the pH range of 6.0-7.5 and produces succinic acid, acetic acid and formic acid at a constant ratio of 2:1:1. When M. succiniciproducensMBEL55E was cultured anaerobically in medium containing 20 g l(-1) glucose as carbon source, 13.5 g l(-1) of succinic acid was produced.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetic Acid / metabolism
  • Animals
  • Carbon Dioxide / metabolism
  • Cattle / microbiology*
  • Fermentation
  • Formates / metabolism
  • Gammaproteobacteria* / classification
  • Gammaproteobacteria* / growth & development
  • Gammaproteobacteria* / isolation & purification
  • Gammaproteobacteria* / physiology
  • Lactic Acid / metabolism
  • Phylogeny
  • RNA, Ribosomal, 16S / genetics
  • Rumen / microbiology*
  • Succinic Acid / metabolism*

Substances

  • Formates
  • RNA, Ribosomal, 16S
  • formic acid
  • Carbon Dioxide
  • Lactic Acid
  • Succinic Acid
  • Acetic Acid