Nicotine administration decreases nitric oxide synthase expression in the hypothalamus of food-deprived rats

Neurosci Lett. 2002 Mar 29;322(1):29-32. doi: 10.1016/s0304-3940(02)00076-9.

Abstract

Effect of nicotine on nitric oxide synthase (NOS) expression in various hypothalamic regions was investigated in rats via nicotineamide adenine dinucleotide phosphate-diaphorase (NADPH-d) histochemistry. Sprague-Dawley rats were divided into the fed group, the fed and nicotine-treated group, the food-deprived group, and the food-deprived and nicotine-treated group. The fed groups received abundant food and water, while food was withheld from the food-deprived groups for 48 h. The nicotine-treated groups were injected with nicotine. Following food deprivation, enhanced NAPDH-d expression was detected in the paraventricular nucleus, ventromedial hypothalamic nucleus, and lateral hypothalamic area of the hypothalamus. Nicotine administration to the food-deprived rats resulted in decreased NADPH-d positivity. The present results indicate that nicotine administration is effective in limiting the enhancement in NOS expression following food restriction.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Appetite Regulation / drug effects
  • Appetite Regulation / physiology
  • Food Deprivation / physiology*
  • Hypothalamic Area, Lateral / cytology
  • Hypothalamic Area, Lateral / drug effects
  • Hypothalamic Area, Lateral / metabolism
  • Hypothalamus / cytology
  • Hypothalamus / drug effects*
  • Hypothalamus / metabolism
  • Immunohistochemistry
  • Male
  • NADPH Dehydrogenase / drug effects
  • NADPH Dehydrogenase / metabolism
  • Nicotine / pharmacology*
  • Nitrergic Neurons / cytology
  • Nitrergic Neurons / drug effects*
  • Nitrergic Neurons / metabolism
  • Nitric Oxide / metabolism*
  • Nitric Oxide Synthase / drug effects*
  • Nitric Oxide Synthase / metabolism
  • Obesity / drug therapy
  • Obesity / metabolism
  • Obesity / physiopathology
  • Paraventricular Hypothalamic Nucleus / cytology
  • Paraventricular Hypothalamic Nucleus / drug effects
  • Paraventricular Hypothalamic Nucleus / metabolism
  • Rats
  • Rats, Sprague-Dawley
  • Tobacco Use Disorder / metabolism*
  • Up-Regulation / drug effects*
  • Up-Regulation / physiology
  • Ventromedial Hypothalamic Nucleus / cytology
  • Ventromedial Hypothalamic Nucleus / drug effects
  • Ventromedial Hypothalamic Nucleus / metabolism

Substances

  • Nitric Oxide
  • Nicotine
  • Nitric Oxide Synthase
  • NADPH Dehydrogenase