Effects of lyophilized black raspberries on azoxymethane-induced colon cancer and 8-hydroxy-2'-deoxyguanosine levels in the Fischer 344 rat

Nutr Cancer. 2001;40(2):125-33. doi: 10.1207/S15327914NC402_8.

Abstract

This study examined the effects of lyophilized black raspberries (BRB) on azoxymethane (AOM)-induced aberrant crypt foci (ACF), colon tumors, and urinary 8-hydroxy-2'-deoxyguanosine (8-OHdG) levels in male Fischer 344 rats. AOM was injected (15 mg/kg body wt i.p.) once per week for 2 wk. At 24 h after the final injection, AOM-treated rats began consuming diets containing 0%, 2.5%, 5%, or 10% (wt/wt) BRB. Vehicle controls received 5% BRB or diet only. Rats were sacrificed after 9 and 33 wk of BRB feeding for ACF enumeration and tumor analysis. ACF multiplicity decreased 36%, 24%, and 21% (P < 0.01 for all groups) in the 2.5%, 5%, and 10% BRB groups, respectively, relative to the AOM-only group. Total tumor multiplicity declined 42%, 45%, and 71% (P < 0.05 for all groups). Although not significant, a decrease in tumor burden (28%, 42%, and 75%) was observed in all BRB groups. Adenocarcinoma multiplicity decreased 28%, 35%, and 80% (P < 0.01) in the same treatment groups. Urinary 8-OHdG levels were reduced by 73%, 81%, and 83% (P < 0.01 for all groups). These results indicate that BRB inhibit several measures of AOM-induced colon carcinogenesis and modulate an important marker of oxidative stress in the Fischer 344 rat.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 8-Hydroxy-2'-Deoxyguanosine
  • Adenocarcinoma / chemically induced
  • Adenocarcinoma / pathology
  • Adenocarcinoma / prevention & control
  • Adenoma / chemically induced
  • Adenoma / pathology
  • Adenoma / prevention & control
  • Animals
  • Anthocyanins / analysis
  • Anticarcinogenic Agents / analysis
  • Azoxymethane*
  • Calcium / analysis
  • Cholesterol / blood
  • Colonic Neoplasms / chemically induced
  • Colonic Neoplasms / pathology
  • Colonic Neoplasms / prevention & control*
  • Coumaric Acids / analysis
  • Deoxyguanosine / analogs & derivatives*
  • Deoxyguanosine / urine*
  • Diet
  • Ellagic Acid / analysis
  • Freeze Drying*
  • Fruit
  • Oxidative Stress
  • Rats
  • Rats, Inbred F344
  • Rosaceae* / chemistry
  • Sitosterols / analysis

Substances

  • Anthocyanins
  • Anticarcinogenic Agents
  • Coumaric Acids
  • Sitosterols
  • Ellagic Acid
  • gamma-sitosterol
  • 8-Hydroxy-2'-Deoxyguanosine
  • Cholesterol
  • ferulic acid
  • Deoxyguanosine
  • Azoxymethane
  • Calcium