Abstract
In eukaryotic cells, secretion is achieved by vesicular transport. Fusion of such vesicles with the correct target compartment relies on SNARE proteins on both vesicle (v-SNARE) and the target membranes (t-SNARE). At present it is not clear how v-SNAREs are incorporated into transport vesicles. Here, we show that binding of ADP-ribosylation factor (ARF)-GTPase-activating protein (GAP) to ER-Golgi v-SNAREs is an essential step for recruitment of Arf1p and coatomer, proteins that together form the COPI coat. ARF-GAP acts catalytically to recruit COPI components. Inclusion of v-SNAREs into COPI vesicles could be mediated by direct interaction with the coat. The mechanisms by which v-SNAREs interact with COPI and COPII coat proteins seem to be different and may play a key role in determining specificity in vesicle budding.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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ADP-Ribosylation Factor 1 / metabolism
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ADP-Ribosylation Factors / metabolism*
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COP-Coated Vesicles / metabolism
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Coat Protein Complex I / metabolism*
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DNA-Binding Proteins / metabolism
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Endoplasmic Reticulum / metabolism*
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Fungal Proteins / metabolism
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GTPase-Activating Proteins / metabolism*
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Intracellular Membranes / metabolism
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Membrane Proteins / metabolism*
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Microsomes / metabolism
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Protein Binding
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Recombinant Fusion Proteins / metabolism
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SNARE Proteins
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Saccharomyces cerevisiae Proteins*
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Vesicular Transport Proteins*
Substances
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Coat Protein Complex I
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DNA-Binding Proteins
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Fungal Proteins
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GCS1 protein, S cerevisiae
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GTPase-Activating Proteins
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Membrane Proteins
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Recombinant Fusion Proteins
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SNARE Proteins
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Saccharomyces cerevisiae Proteins
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Vesicular Transport Proteins
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ADP-Ribosylation Factor 1
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ADP-Ribosylation Factors