Abstract
An interferon-stimulated response element (ISRE)/interferon regulatory element (IRE) spanning nucleotide coordinates 1091-1100 is present in the enhancer 1/X gene promoter region of the hepatitis B virus (HBV) genome. In the context of a minimal promoter element, the enhancer 1/X gene promoter ISRE/IRE was shown to be a functional regulatory site capable of mediating interferon alpha- (IFNalpha) and interferon-stimulated gene factor 3 (ISGF3)-specific transcriptional activation in transient transfection analysis. The enhancer 1/X gene promoter ISRE/IRE was also shown to mediate interferon regulatory factor (IRF) 1 and IRF7 activation of transcription from a minimal promoter construct. In contrast, IFNalpha and the IRFs had minimal effect on HBV transcription and replication in the context of the viral genome in cell culture.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Base Sequence
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DNA, Viral / genetics
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DNA-Binding Proteins / physiology
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Enhancer Elements, Genetic*
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Gene Expression Regulation, Viral*
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Genome, Viral
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Hepatitis B / genetics*
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Hepatitis B / metabolism
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Humans
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Interferon Regulatory Factor-1
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Interferon Regulatory Factor-7
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Interferon-Stimulated Gene Factor 3
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Interferon-Stimulated Gene Factor 3, gamma Subunit
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Interferon-alpha / pharmacology*
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Molecular Sequence Data
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Phosphoproteins / physiology
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Promoter Regions, Genetic
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RNA, Viral / biosynthesis
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Response Elements*
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Transcription Factors / physiology
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Transcriptional Activation
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Tumor Cells, Cultured
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Virus Replication
Substances
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DNA, Viral
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DNA-Binding Proteins
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IRF1 protein, human
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IRF7 protein, human
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IRF9 protein, human
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Interferon Regulatory Factor-1
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Interferon Regulatory Factor-7
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Interferon-Stimulated Gene Factor 3
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Interferon-Stimulated Gene Factor 3, gamma Subunit
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Interferon-alpha
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Phosphoproteins
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RNA, Viral
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Transcription Factors