Significant up-regulation of a novel gene, CLCP1, in a highly metastatic lung cancer subline as well as in lung cancers in vivo

Oncogene. 2002 Apr 25;21(18):2822-8. doi: 10.1038/sj.onc.1205405.


Most lung cancer patients are unfortunately uncurable and die because of widespread metastases, thus indicating the importance of identification of molecules with a crucial role in this process. Our previous expression profiling analysis of a highly metastatic lung cancer cell line, NCI-H460-LNM35, and its parental low metastatic line, NCI-H460-N15, revealed significant up-regulation of both known and unknown genes in LNM35. In this study, we describe the isolation and detailed characterizations of a novel gene, named CLCP1, which corresponds to one of such expression sequence tags with up-regulated expression in LNM35. The CLCP1 gene was found to encode a protein with 775 amino acids with structural similarities to, but distinct from neuropilins, cell surface receptors for VEGF165 and semaphorins. Notably, CLCP1 was shown to be up-regulated not only in LNM35 in association with its acquisition of metastatic phenotype during in vivo selection, but also in a significant fraction of lung cancers in vivo with high frequency in metastatic lesions, warranting future studies for a better understanding of the molecular mechanisms of lung cancer metastasis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenocarcinoma / genetics*
  • Adenocarcinoma / pathology
  • Adult
  • Amino Acid Sequence
  • Base Sequence
  • Carcinoma, Large Cell / genetics*
  • Carcinoma, Large Cell / pathology
  • Carcinoma, Small Cell / genetics*
  • Carcinoma, Small Cell / pathology
  • Carcinoma, Squamous Cell / genetics*
  • Carcinoma, Squamous Cell / pathology
  • Chemical Fractionation
  • Cloning, Molecular
  • DNA, Complementary
  • Gene Expression Regulation, Neoplastic*
  • Humans
  • Lung Neoplasms / genetics*
  • Lung Neoplasms / pathology
  • Membrane Proteins / genetics*
  • Molecular Sequence Data
  • Neoplasm Metastasis
  • Neoplasm Proteins / genetics*
  • Sequence Homology, Nucleic Acid
  • Transfection
  • Tumor Cells, Cultured
  • Up-Regulation*


  • DCBLD2 protein, human
  • DNA, Complementary
  • Membrane Proteins
  • Neoplasm Proteins

Associated data

  • GENBANK/AB073146
  • GENBANK/AF387547