Integramides A and B, two novel non-ribosomal linear peptides containing nine C(alpha)-methyl amino acids produced by fungal fermentations that are inhibitors of HIV-1 integrase

Org Lett. 2002 May 2;4(9):1431-4. doi: 10.1021/ol025540a.

Abstract

[structure: see text]. Integramides A and B are two novel 16-mer linear peptides rich in C(alpha)-methyl amino acids that were isolated from fungal extracts of Dendrodochium sp. by employing a bioassay-guided isolation procedure using recombinant HIV-1 integrase. The structure and stereochemistry were elucidated by a combination of 2D NMR and ESI- and FAB-MS including MS/MS studies and by Marfey's method. Integramides A and B inhibited the coupled reaction of HIV-1 integrase with IC50 values of 17 and 10 microM, respectively.

MeSH terms

  • Chromatography, High Pressure Liquid
  • Fermentation
  • Fungi / metabolism
  • HIV Integrase / chemistry*
  • HIV Integrase Inhibitors / chemical synthesis*
  • Oligopeptides / chemistry
  • Peptides / chemistry*

Substances

  • HIV Integrase Inhibitors
  • Oligopeptides
  • Peptides
  • integramide A
  • integramide B
  • HIV Integrase