H-2 haplotype-dependent serum IL-12 production in tumor-bearing mice treated with various mycelial extracts

In Vivo. Jan-Feb 2002;16(1):49-54.

Abstract

IL-12 is considered to be one of the most important cytokines in anti-cancer therapy. We have demonstrated that substances derived from Basidiomycetes, such as active hexose-correlated compound (AHCC) and PSK induce the production of IL-12. In this study, the MHC dependency of IL-12 production induced by various mycelial extracts, PSK, AHCC and IL-X, was examined. During tumor-bearing, higher serum IL-12 levels were observed in H-2a and H-2b mice as compared to H-2d mice. Concerning the effect of genetic background of mice on response to mycelial extracts, AHCC administration enhanced the serum IL-12 level in H-2b mice but not in H-2d mice, while PSK administration increased the serum IL-12 level in H-2d mice but not in H-2b mice. IL-X, components derived from the same Basidiomycetes, also enhanced the serum IL-12 level in H-2b mice in the early stage of tumor like AHCC, and maintained serum IL-12 at a level higher than the normal value accompanying tumor growth, whereas AHCC did not restore the lowered serum IL-12 level accompanying tumor growth. These results showed that AHCC or IL-X is effective in a genetically Th1-dominant individual whereas PSK is effective in a genetically Th2-dominant individual or Th2-dominant status in advanced cancer patients. So we propose that the suitable combinations of various mycelial extracts may be effective methods of endogenous IL-12 induction for cancer patients of all stages, which is important as a cancer therapy that is relatively free from adverse reactions and which emphasizes the QOL in individual patients.

MeSH terms

  • Adenocarcinoma
  • Adjuvants, Immunologic / chemistry
  • Adjuvants, Immunologic / therapeutic use
  • Animals
  • Antineoplastic Agents / chemistry
  • Antineoplastic Agents / therapeutic use*
  • Basidiomycota* / chemistry
  • Carcinoma, Lewis Lung
  • Colonic Neoplasms
  • H-2 Antigens / immunology*
  • Haplotypes
  • Interleukin-12 / blood*
  • Mice
  • Mice, Congenic
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Mycelium / chemistry
  • Neoplasms, Experimental* / blood
  • Neoplasms, Experimental* / drug therapy
  • Neoplasms, Experimental* / immunology
  • Plant Extracts / chemistry
  • Plant Extracts / therapeutic use*
  • Proteoglycans / chemistry
  • Proteoglycans / therapeutic use

Substances

  • Adjuvants, Immunologic
  • Antineoplastic Agents
  • H-2 Antigens
  • Plant Extracts
  • Proteoglycans
  • Interleukin-12
  • polysaccharide-K