Neonatal dendritic cells are intrinsically biased against Th-1 immune responses

Clin Exp Immunol. 2002 Apr;128(1):118-23. doi: 10.1046/j.1365-2249.2002.01817.x.

Abstract

Dendritic cells (DCs) were derived from human peripheral blood monocytes or cord blood monocytes cultured in the presence of IL-4 and GM-CSF. Adult and cord DCs were observed to have comparable immature phenotypes. However, the increase in surface expression of HLA-DR and CD86 after addition of LPS was significantly attenuated in cord DCs, with CD25 and CD83 expression also markedly reduced. Cord DCs were also unable to produce IL-12p70, failed to down-regulate expression of the chemokine receptor CCR5 and induced lower levels of IFN-gamma production from allogeneic naive CD4+ T cells than their adult counterparts. In contrast, the kinetics of the production of TNF-alpha and IL-10 in response to LPS stimulation was comparable to adult DCs. The reduced ability of cord DCs to attain a fully mature adult phenotype, and to activate naive CD4+ T cells to produce IFN-gamma, suggests that they are intrinsically preprogrammed against the generation of Th-1 immune responses.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Antigen Presentation*
  • CD4-Positive T-Lymphocytes / immunology
  • Cell Differentiation
  • Cells, Cultured
  • Dendritic Cells / classification
  • Dendritic Cells / drug effects
  • Dendritic Cells / immunology*
  • Fetal Blood / cytology
  • Fetal Blood / immunology
  • Humans
  • Immunophenotyping
  • Infant, Newborn
  • Interleukin-10 / biosynthesis
  • Isoantigens / immunology
  • Kinetics
  • Lipopolysaccharides / pharmacology
  • Th1 Cells / immunology*
  • Tumor Necrosis Factor-alpha / biosynthesis

Substances

  • Isoantigens
  • Lipopolysaccharides
  • Tumor Necrosis Factor-alpha
  • Interleukin-10