Activator-specific recruitment of TFIID and regulation of ribosomal protein genes in yeast

Mol Cell. 2002 Apr;9(4):823-33. doi: 10.1016/s1097-2765(02)00490-2.


In yeast, TFIID strongly associates with nearly all ribosomal protein (RP) promoters, but a TAF-independent form of TBP preferentially associates with other active promoters. RP promoters are regulated in response to growth stimuli, in most cases by a Rap1-containing activator. This Rap1-dependent activator is necessary and sufficient for TFIID recruitment, whereas other activators do not efficiently recruit TFIID. TAFs are recruited to RP promoters even when TBP and other general transcription factors are not associated, suggesting that TFIID recruitment involves a direct activator-TAF interaction. Most RP promoters lack canonical TATA elements, and they are preferentially activated by the Rap1-containing activator. These results demonstrate activator-specific recruitment of TFIID in vivo, and they suggest that TFIID recruitment is important for coordinate expression of RP genes.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Chromatin / genetics
  • DNA, Fungal / genetics
  • DNA, Fungal / metabolism*
  • Gene Expression Regulation, Fungal / drug effects
  • Glucose / pharmacology
  • Models, Genetic
  • Precipitin Tests
  • Promoter Regions, Genetic / genetics
  • Recombinant Fusion Proteins / physiology
  • Ribosomal Proteins / biosynthesis
  • Ribosomal Proteins / genetics*
  • Saccharomyces cerevisiae / drug effects
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / metabolism*
  • Saccharomyces cerevisiae Proteins / metabolism*
  • TATA Box
  • Trans-Activators / physiology
  • Transcription Factor TFIID
  • Transcription Factors, TFII / metabolism*
  • rap1 GTP-Binding Proteins / physiology


  • Chromatin
  • DNA, Fungal
  • Recombinant Fusion Proteins
  • Ribosomal Proteins
  • Saccharomyces cerevisiae Proteins
  • Trans-Activators
  • Transcription Factor TFIID
  • Transcription Factors, TFII
  • rap1 GTP-Binding Proteins
  • Glucose