Wild-type levels of Spo11-induced DSBs are required for normal single-strand resection during meiosis

Mol Cell. 2002 Apr;9(4):835-46. doi: 10.1016/s1097-2765(02)00498-7.

Abstract

We have studied the repair of a DNA-DSB created by the VMA1-derived endonuclease in mutants that have different levels of Spo11-DSBs: WT (sae2), few (hop1), and none (spo11-Y135F). In spo11-Y135F and hop1 cells, intrachromosomal repair is more frequent than in WT and sae2 cells. In spo11-Y135F cells there was no chromosome pairing or synapsis and a faster turnover of resected DNA. Compared to WT and sae2 cells, spo11-Y135F and hop1 cells have a greater proportion of long resection tracts. The data suggest that high levels of Spo11-DSBs are required for normal regulation of resection, even at a DSB created by another protein. WT control over resection could be important for directing repair to be interchromosomal, increasing the chance of creating interhomolog connections essential to meiotic segregation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromosome Breakage*
  • Chromosomes, Fungal / genetics*
  • Chromosomes, Fungal / ultrastructure
  • DNA Repair
  • DNA, Fungal / genetics*
  • DNA, Fungal / metabolism
  • DNA, Single-Stranded / genetics*
  • DNA, Single-Stranded / metabolism
  • Endodeoxyribonucleases / physiology*
  • Esterases / deficiency
  • Esterases / genetics
  • Esterases / physiology*
  • Meiosis / genetics*
  • Models, Genetic
  • Proton-Translocating ATPases*
  • Recombination, Genetic / physiology*
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae Proteins / genetics
  • Saccharomyces cerevisiae Proteins / physiology*
  • Sequence Deletion
  • Synaptonemal Complex / physiology

Substances

  • DNA, Fungal
  • DNA, Single-Stranded
  • Saccharomyces cerevisiae Proteins
  • Endodeoxyribonucleases
  • Esterases
  • meiotic recombination protein SPO11
  • Proton-Translocating ATPases
  • VMA1 protein, S cerevisiae