Meiotic DNA breaks at the S. pombe recombination hot spot M26

Mol Cell. 2002 Apr;9(4):847-55. doi: 10.1016/s1097-2765(02)00489-6.

Abstract

The ade6-M26 allele of Schizosaccharomyces pombe creates a well-defined meiotic recombination hot spot that requires a specific sequence, 5'-ATGACGT-3', and the Atf1*Pcr1 transcription factor for activity. We find that M26 stimulates the formation of meiosis-specific double-strand DNA breaks at multiple sites surrounding M26. Like hot spot activity, breakage requires the M26 heptamer, Pcr1, and the general recombination factor Rec12. When the M26 heptamer is moved to new positions within ade6, new break sites are observed spanning approximately 0.5-2 kb around the moved heptamer. Break frequency is strongly correlated with recombination frequency for these alleles. The occurrence of breaks at M26 suggests mechanistic similarities to hot spots in the distantly related yeast Saccharomyces cerevisiae.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Activating Transcription Factor 1
  • Activating Transcription Factors
  • Alleles
  • Carboxy-Lyases / genetics*
  • Chromosome Breakage*
  • Chromosomes, Fungal / genetics*
  • Chromosomes, Fungal / ultrastructure
  • DNA Repair
  • DNA, Fungal / genetics*
  • DNA, Fungal / metabolism
  • DNA-Binding Proteins / deficiency
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / physiology*
  • Meiosis / genetics*
  • RNA-Binding Proteins
  • Recombination, Genetic / genetics*
  • Regulatory Sequences, Nucleic Acid
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae Proteins*
  • Schizosaccharomyces / genetics*
  • Schizosaccharomyces pombe Proteins / genetics
  • Schizosaccharomyces pombe Proteins / physiology*
  • Species Specificity
  • Temperature
  • Transcription Factors / deficiency
  • Transcription Factors / genetics
  • Transcription Factors / physiology*

Substances

  • Activating Transcription Factor 1
  • Activating Transcription Factors
  • DNA, Fungal
  • DNA-Binding Proteins
  • PAT1 protein, S cerevisiae
  • Pcr1 protein, S pombe
  • RNA-Binding Proteins
  • Saccharomyces cerevisiae Proteins
  • Schizosaccharomyces pombe Proteins
  • Transcription Factors
  • rec12 protein, S pombe
  • Carboxy-Lyases
  • phosphoribosylaminoimidazole carboxylase