Neuronal Differentiation of Cultured Human NTERA-2cl.D1 Cells Leads to Increased Expression of Synapsins

Neurosci Lett. 2002 May 10;324(1):37-40. doi: 10.1016/s0304-3940(02)00170-2.

Abstract

The synapsin family consists of three neuronal-specific phosphoproteins associated with dynamic reorganization of the neuronal cytoskeleton. Synapsin I and II are implicated in axonal and synaptic differentiation, formation and maintenance, whereas the function of synapsin III is not as well defined. We report a significant transcriptional upregulation of all three synapsins (synapsin I, 2.1-fold; synapsin II, 2.6-fold; and synapsin III, 5.5-fold) by retinoic acid-induced differentiation of NTera-2cl.D1 cells, a human paradigm for neuronal differentiation. The observed stronger regulation of synapsin III might be due to still active neurite elongation and a rather early state of presynaptic maturation at the time-point investigated, as synapsin III was previously found to be highly enriched in growth cones and during early synaptic development.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Cell Aggregation / drug effects
  • Cell Aggregation / physiology
  • Cell Differentiation / drug effects
  • Cell Differentiation / physiology*
  • Cell Movement / drug effects
  • Cell Movement / physiology
  • Central Nervous System / cytology
  • Central Nervous System / embryology*
  • Central Nervous System / metabolism*
  • Culture Techniques / methods
  • Gene Expression Regulation, Developmental / drug effects
  • Gene Expression Regulation, Developmental / physiology*
  • Growth Cones / drug effects
  • Growth Cones / metabolism
  • Growth Cones / ultrastructure
  • Humans
  • Models, Biological
  • Neural Pathways / cytology
  • Neural Pathways / embryology
  • Neural Pathways / metabolism
  • Neurites / drug effects
  • Neurites / metabolism
  • Neurites / ultrastructure
  • Neurofilament Proteins / drug effects
  • Neurofilament Proteins / metabolism
  • Neurons / cytology
  • Neurons / drug effects
  • Neurons / metabolism*
  • Neuropeptides / drug effects
  • Neuropeptides / metabolism
  • Phosphoproteins / drug effects
  • Phosphoproteins / metabolism
  • Synapses / drug effects
  • Synapses / metabolism
  • Synapses / ultrastructure
  • Synapsins / drug effects
  • Synapsins / metabolism*
  • Teratocarcinoma
  • Tretinoin / pharmacology
  • Tumor Cells, Cultured
  • Up-Regulation / drug effects
  • Up-Regulation / physiology*

Substances

  • Neurofilament Proteins
  • Neuropeptides
  • Phosphoproteins
  • SYN3 protein, human
  • Synapsins
  • neurofilament protein M
  • Tretinoin