Relationship between physical activity and oxidative stress biomarkers in women

Med Sci Sports Exerc. 2002 May;34(5):814-9. doi: 10.1097/00005768-200205000-00014.


Purpose: To examine the relationship between physical activity and levels of plasma lipid peroxides, superoxide dismutase in erythrocytes (SOD), and glutathione peroxidase (GSH-Px) in whole blood activities.

Methods: Cross-sectional study in 488 Spanish women. Two categories of leisure time physical activity were defined according to their intensity: low (<or=6 METs) and high (>6 METs). Energy expenditure in household activities was also recorded. Multivariate linear regression analyses were used to adjust for the effect of physical activity on lipid peroxides and SOD and GSH-Px for confounding variables.

Results: The amount of leisure time physical activity was associated with high activity levels of SOD (P = 0.022) and GSH-Px (P = 0.002). Similar results were obtained when physical activity in household activities was added to total leisure physical activity. Physical activity of low intensity was associated with high SOD activity levels (P = 0.002) and that of high intensity with high GSH-Px activity levels (P = 0.001).

Conclusion: The amount and intensity of leisure physical activity were directly related to both antioxidant enzyme activity levels. The findings of this study suggest a modulatory effect of leisure physical activity intensity on antioxidative balance in the studied female population.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Analysis of Variance
  • Biomarkers / blood*
  • Exercise / physiology*
  • Exercise Test
  • Female
  • Glutathione Peroxidase / blood
  • Humans
  • Life Style
  • Lipid Peroxides / blood
  • Middle Aged
  • Oxidative Stress / physiology
  • Smoking
  • Superoxide Dismutase / blood


  • Biomarkers
  • Lipid Peroxides
  • Glutathione Peroxidase
  • Superoxide Dismutase