A chromodomain-containing nuclear protein, MRG15 is expressed as a novel type of dendritic mRNA in neurons

Neurosci Res. 2002 Apr;42(4):299-308. doi: 10.1016/s0168-0102(02)00010-x.

Abstract

On the basis of a hypothesis that proteins encoded by the mRNAs that are transported to and translated at the dendrites/synapses may play key roles in synaptic plasticity, this study reports on attempts to isolate mRNAs which are localizing at the dendrites/synapses from mouse cerebellar synaptosomal fractions. Among 100 pieces of dendritic mRNA candidates, 10 pieces of mRNAs were found to contain the cytoplasmic polyadenylation element (CPE)-like sequences which were contained in certain mRNAs translated in dendrites. We next examined the issue of whether the CPE-like sequence-containing mRNAs (CPERs) were localized in the synapses/dendrites by means of in situ hybridization. The findings indicate that CPER9 was actually localized at the apical dendrites of a portion of cerebral cortex layer V pyramidal cells, as well as at the proximal dendrites of some of the cerebellar Purkinje cells. CPER9 was found to encode a mouse homolog of MRG15, a nuclear protein which contains a chromodomain identified in several proteins that act as regulators of transcription. Immunohistochemistry with anti-MRG15 antibodies revealed that MRG15 was localized in dendrites as well as in the nuclei of Purkinje cells. These results suggest that MRG15 may serve as a link between synaptic activity and gene expression.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibody Specificity / immunology
  • Base Sequence / genetics
  • Brain / cytology
  • Brain / growth & development*
  • Brain / metabolism
  • Cell Compartmentation / physiology
  • Cerebellar Cortex / cytology
  • Cerebellar Cortex / metabolism
  • Cerebral Cortex / cytology
  • Cerebral Cortex / metabolism
  • Dendrites / metabolism*
  • Dendrites / ultrastructure
  • Gene Expression / physiology
  • Immunohistochemistry
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Nuclear Proteins / genetics*
  • Nuclear Proteins / metabolism*
  • Presynaptic Terminals / metabolism*
  • Presynaptic Terminals / ultrastructure
  • Protein Structure, Tertiary / genetics
  • Purkinje Cells / cytology
  • Purkinje Cells / metabolism
  • Pyramidal Cells / cytology
  • Pyramidal Cells / metabolism
  • RNA, Messenger / genetics
  • RNA, Messenger / isolation & purification*
  • RNA-Binding Proteins / genetics
  • RNA-Binding Proteins / metabolism
  • Sequence Homology, Amino Acid
  • Transcription Factors / genetics
  • Transcription Factors / metabolism
  • mRNA Cleavage and Polyadenylation Factors*

Substances

  • Cpeb1 protein, mouse
  • MORF4 protein, human
  • Nuclear Proteins
  • RNA, Messenger
  • RNA-Binding Proteins
  • Transcription Factors
  • mRNA Cleavage and Polyadenylation Factors