GATEWAY vectors for Agrobacterium-mediated plant transformation

Trends Plant Sci. 2002 May;7(5):193-5. doi: 10.1016/s1360-1385(02)02251-3.


Agrobacterium tumefaciens is the preferred method for transformation of a wide range of plant species. Commonly, the genes to be transferred are cloned between the left and right T-DNA borders of so-called binary T-DNA vectors that can replicate both in E. coli and Agrobacterium. Because these vectors are generally large, cloning can be time-consuming and laborious. Recently, the GATEWAY conversion technology has provided a fast and reliable alternative to the cloning of sequences into large acceptor plasmids.

MeSH terms

  • Agrobacterium tumefaciens / genetics*
  • DNA, Bacterial / genetics
  • Escherichia coli / genetics
  • Genetic Vectors / genetics*
  • Plants, Genetically Modified / genetics*
  • Plasmids / genetics


  • DNA, Bacterial
  • T-DNA