Abstract
The analysis of several Ly49 genes has identified a tissue-specific promoter adjacent to the previously defined first exon. The current study reveals the presence of an additional Ly49 promoter (Pro-1) and two noncoding exons upstream of the previously defined promoter (Pro-2). DNA sequences homologous to Pro-1 are present 4-10 kb upstream of Pro-2 in all Ly49 genes examined, and Pro-1 transcripts were detected from the Ly49a, e, g, o, and v genes. Pro-1 activity can be detected in bone marrow, embryonic thymus, freshly isolated liver NK cells, and the murine LNK cell line, but it does not function in adult thymus, sorted NK-T cells, spleen NK cells, or the EL-4 T cell line, even though these cells express Ly49 proteins. Luciferase reporter assays identified a Pro-1 core promoter region that functions in the LNK cell line but not EL-4 cells. The novel promoter is not active in mature NK cells, suggesting that Pro-1 represents an early Ly49 promoter.
Publication types
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Comparative Study
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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5' Untranslated Regions / immunology
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Alternative Splicing / immunology
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Animals
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Antigens, Ly / genetics*
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Base Sequence
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Bone Marrow Cells / immunology*
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Bone Marrow Cells / metabolism*
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Cell Line
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Exons / immunology
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Fetus
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Gene Expression Regulation / immunology*
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Killer Cells, Natural
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Lectins, C-Type
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Liver / cytology
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Liver / immunology
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Membrane Glycoproteins / genetics*
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Mice
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Mice, Inbred BALB C
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Mice, Inbred C57BL
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Molecular Sequence Data
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Multigene Family / immunology
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NK Cell Lectin-Like Receptor Subfamily A
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Promoter Regions, Genetic / immunology*
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RNA, Messenger / isolation & purification
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Receptors, NK Cell Lectin-Like
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Thymus Gland / immunology*
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Thymus Gland / metabolism*
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Transcription Initiation Site
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Transcription, Genetic / immunology
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Tumor Cells, Cultured
Substances
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5' Untranslated Regions
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Antigens, Ly
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Klra1 protein, mouse
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Lectins, C-Type
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Membrane Glycoproteins
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NK Cell Lectin-Like Receptor Subfamily A
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RNA, Messenger
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Receptors, NK Cell Lectin-Like
Associated data
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GENBANK/AF419249
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GENBANK/AF421168