cDNA Cloning and Characterization of Tobacco ABC Transporter: NtPDR1 Is a Novel Elicitor-Responsive Gene

FEBS Lett. 2002 May 8;518(1-3):164-8. doi: 10.1016/s0014-5793(02)02697-2.

Abstract

We isolated an INF1 elicitin-inducible cDNA encoding a pleiotropic drug resistance (PDR)-type ATP-binding cassette (ABC) transporter homolog (NtPDR1) in suspension-cultured tobacco Bright Yellow-2 (BY-2) cells by application of differential display PCR. The NtPDR1 (Nicotiana tabacum PDR protein 1) gene also encodes a 162 kDa protein that includes two putative hydrophilic domains containing the ABC signature motif and two putative hydrophobic domains. Expression of the NtPDR1 gene was rapidly and strongly activated by treatment of BY-2 cells with INF1 elicitin. Further, treatment of BY-2 cells with flagellin, a bacterial proteinaceous hypersensitive reaction elicitor, or yeast extract, a general elicitor, also induced NtPDR1 gene expression. These results indicate that NtPDR1 may be involved in the general defense response in tobacco. This is the first report that microbial elicitors induce the expression of a plant ABC transporter gene.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • ATP-Binding Cassette Transporters / biosynthesis*
  • ATP-Binding Cassette Transporters / chemistry
  • ATP-Binding Cassette Transporters / genetics*
  • Algal Proteins
  • Amino Acid Sequence
  • Cells, Cultured
  • Cloning, Molecular
  • DNA, Complementary
  • Flagellin / pharmacology
  • Fungal Proteins / pharmacology
  • Gene Expression Regulation, Plant*
  • Kinetics
  • Molecular Sequence Data
  • Plant Growth Regulators / pharmacology
  • Plant Proteins / biosynthesis*
  • Plant Proteins / chemistry
  • Plant Proteins / genetics*
  • Protein Structure, Tertiary
  • Proteins
  • RNA, Plant / biosynthesis
  • Sequence Alignment
  • Tobacco / drug effects
  • Tobacco / genetics
  • Tobacco / metabolism*
  • Transcriptional Activation

Substances

  • ATP-Binding Cassette Transporters
  • Algal Proteins
  • DNA, Complementary
  • Fungal Proteins
  • Plant Growth Regulators
  • Plant Proteins
  • Proteins
  • RNA, Plant
  • elicitin, Phytophthora
  • Flagellin

Associated data

  • GENBANK/AB075550