The role of the anterior neural ridge and Fgf-8 in early forebrain patterning and regionalization in Xenopus laevis

Comp Biochem Physiol B Biochem Mol Biol. 2002 May;132(1):179-89. doi: 10.1016/s1096-4959(01)00521-8.

Abstract

The tissue, cellular and molecular mechanisms that regulate early regional specification of the vertebrate forebrain are largely unknown. We studied the expression patterns of Xbf-1, an anterior (and telencephalon) neural-specific winged helix transcription factor and Fgf-8, an early-secreted factor. This study looked at Xbf-1 and Fgf-8 expression in combination with embryonic grafting experiments and also used beads containing the recombinant Fgf-8 protein to determine these factors' effects upon forebrain patterning events. We provide evidence that additional Fgf-8 displaces Xbf-1 expression posteriorly, suggesting a concentration dependence of Fgf-8 for the early distinct regionalization of the telencephalic primordia. Also, additional stage 15 mid-anterior neural ridge (mANR) transplants inhibited telencephalon development, whereas lateral ANR transplants facilitated increased areas of telencephalon development. In both cases, these transplantations promoted ectopic expression of Xbf-1. These studies suggested that the distinct regionalization of the forebrain primordia involves the inhibitory actions of the mANR towards a telencephalon development and maintaining bilateral telencephali. These telencephalic primordia are initially localized by optimal Fgf-8 expression. The anterior mANR will eventually become the anterior and rostral diencephalic tissue. This in vivo study demonstrated Fgf-8 and the mANR are important in forebrain regionalization.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Body Patterning
  • Fibroblast Growth Factor 8
  • Fibroblast Growth Factors / biosynthesis*
  • Fibroblast Growth Factors / physiology*
  • In Situ Hybridization
  • Neural Crest / embryology*
  • Neurons / metabolism
  • Prosencephalon / embryology*
  • RNA, Messenger / metabolism
  • Time Factors
  • Transcription Factors / biosynthesis
  • Xenopus Proteins*
  • Xenopus laevis / embryology*

Substances

  • FOXG1 protein, Xenopus
  • RNA, Messenger
  • Transcription Factors
  • Xenopus Proteins
  • Fibroblast Growth Factor 8
  • Fibroblast Growth Factors