Abstract
Apolipoprotein E is the predominant brain lipoprotein and polymorphic variation in the APOE gene the major genetic susceptibly factor for late onset Alzheimer's disease (AD). Recently it was reported that carboxyl-truncated ApoE fragments induce tangle-like structures in neurons. We confirm the finding: in mouse neuroblastoma cells truncated apoE fragments lacking the carboxyterminus induce structures that have the appearance of neurofibrillary tangles. However these tangles are not induced in non-neuronal cells even in the presence of co-expressed neurofilaments or tau. Further understanding of the basis of this cell specificity might add to understanding of the cell specificity of tangles in AD.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alzheimer Disease / metabolism*
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Alzheimer Disease / physiopathology
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Animals
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Apolipoproteins E / deficiency*
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Apolipoproteins E / genetics*
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Brain / metabolism*
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Brain / pathology
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Brain / physiopathology
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CHO Cells
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COS Cells
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Cricetinae
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DNA, Complementary / genetics
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Fetus
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Immunohistochemistry
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Inclusion Bodies / metabolism
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Mice
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Neurofibrillary Tangles / metabolism*
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Neurofibrillary Tangles / pathology
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Neurofilament Proteins / genetics
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Neurofilament Proteins / metabolism
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Neurons / metabolism*
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Neurons / pathology
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Peptide Fragments / metabolism*
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Protein Sorting Signals / genetics
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Protein Structure, Tertiary / physiology
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Transport Vesicles / metabolism
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Tumor Cells, Cultured
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tau Proteins / metabolism
Substances
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Apolipoproteins E
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DNA, Complementary
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Neurofilament Proteins
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Peptide Fragments
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Protein Sorting Signals
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tau Proteins