Monitoring large-scale changes in transcript abundance in drought- and salt-stressed barley

Plant Mol Biol. Mar-Apr 2002;48(5-6):551-73. doi: 10.1023/a:1014875215580.

Abstract

Responses to drought and salinity in barley (Hordeum vulgare L. cv. Tokak) were monitored by microarray hybridization of 1463 DNA elements derived from cDNA libraries of 6 and 10 h drought-stressed plants. Functional identities indicated that many cDNAs in these libraries were associated with drought stress. About 38% of the transcripts were novel and functionally unknown. Hybridization experiments were analyzed for drought- and salinity-regulated sequences, with significant changes defined as a deviation from the control exceeding 2.5-fold. Responses of transcripts showed stress-dependent expression patterns and time courses. Nearly 15% of all transcripts were either up- or down-regulated under drought stress, while NaCl led to a change in 5% of the transcripts (24 h, 150 mM NaCl). Transcripts that showed significant up-regulation under drought stress are exemplified by jasmonate-responsive, metallothionein-like, late-embryogenesis-abundant (LEA) and ABA-responsive proteins. Most drastic down-regulation in a category was observed for photosynthesis-related functions. Up-regulation under both drought and salt stress was restricted to ESTs for metallothionein-like and LEA proteins, while increases in ubiquitin-related transcripts characterized salt stress. A number of functionally unknown transcripts from cDNA libraries of drought-stressed plants showed up-regulation by drought but down-regulation by salt stress, documenting how precisely transcript profiles report different growth conditions and environments.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Disasters
  • Down-Regulation / drug effects
  • Gene Expression Regulation, Plant / drug effects
  • Gene Library
  • Genes, Plant / genetics
  • Hordeum / drug effects*
  • Hordeum / genetics
  • Hordeum / growth & development
  • Oligonucleotide Array Sequence Analysis / methods
  • Plant Roots / drug effects
  • Plant Roots / genetics
  • Plant Roots / growth & development
  • RNA, Plant / genetics
  • RNA, Plant / metabolism
  • Sodium Chloride / pharmacology*
  • Transcription, Genetic
  • Up-Regulation / drug effects
  • Water / pharmacology*

Substances

  • RNA, Plant
  • Water
  • Sodium Chloride