Comparative assessment of large-scale data sets of protein-protein interactions

Nature. 2002 May 23;417(6887):399-403. doi: 10.1038/nature750. Epub 2002 May 8.


Comprehensive protein protein interaction maps promise to reveal many aspects of the complex regulatory network underlying cellular function. Recently, large-scale approaches have predicted many new protein interactions in yeast. To measure their accuracy and potential as well as to identify biases, strengths and weaknesses, we compare the methods with each other and with a reference set of previously reported protein interactions.

Publication types

  • Comparative Study
  • Evaluation Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Bias
  • Databases, Factual
  • False Positive Reactions
  • Fungal Proteins / genetics
  • Fungal Proteins / metabolism
  • Mass Spectrometry
  • Protein Binding
  • Proteins / genetics
  • Proteins / metabolism*
  • RNA, Messenger / analysis
  • RNA, Messenger / genetics
  • Reference Standards
  • Sensitivity and Specificity
  • Two-Hybrid System Techniques


  • Fungal Proteins
  • Proteins
  • RNA, Messenger