Development of dioxin toxicity evaluation method in human milk by enzyme-linked immunosorbent assay--assay validation for human milk

Chemosphere. 2002 Mar;46(9-10):1471-6. doi: 10.1016/s0045-6535(01)00267-3.

Abstract

In this study, the development of a toxicity evaluation method for dioxins in human milk by enzyme-linked immunosorbent assay (ELISA) was reported. A total of 17 human milk samples were tested by ELISA and by gas chromatography/mass spectrometry (GC/MS) to assess whether the ELISA performed on samples obtained from primiparas could be considered as reliable enough for identifying a dioxins contamination in human milk. The concept of toxicity equivalent quantity (TEQ) screening was validated by comparing TEQ values for a set of human milk samples to the ELISA responses predicted for those samples. A fairly good correlation (r = 0.920) between immunoassay and GC/MS was achieved for human milk. This ELISA should be useful for biological samples monitoring.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adult
  • Benzofurans / analysis
  • Benzofurans / toxicity*
  • Dibenzofurans, Polychlorinated
  • Enzyme-Linked Immunosorbent Assay / methods*
  • Female
  • Forecasting
  • Gas Chromatography-Mass Spectrometry
  • Humans
  • Milk, Human / chemistry*
  • Models, Theoretical*
  • Polychlorinated Dibenzodioxins / analogs & derivatives*
  • Polychlorinated Dibenzodioxins / analysis
  • Polychlorinated Dibenzodioxins / toxicity*
  • Reference Values
  • Soil Pollutants / analysis
  • Soil Pollutants / toxicity*

Substances

  • Benzofurans
  • Dibenzofurans, Polychlorinated
  • Polychlorinated Dibenzodioxins
  • Soil Pollutants