Alteration of the LRP1B gene region is associated with high grade of urothelial cancer

Lab Invest. 2002 May;82(5):639-43. doi: 10.1038/labinvest.3780458.

Abstract

We have delineated regions of interest at chromosome 2q21.2, 2q36.3, and 2q37.1 by deletion mapping of 114 urothelial cancers (UC). Altogether, 17%, 18%, and 63% of the G1, G2, and G3 tumors displayed loss of heterozygosity at chromosome 2q, respectively, The region at 2q21.2 was narrowed down to the LRP1B gene (NT_005129.6). Hemi- and homozygous deletion at the LRP1B gene region was seen in 31 of 114 UCs. Only 8% of the UCs with G1 and none with G2 tumors showed loss of heterozygosity at the LRP1B gene, whereas 49% of the G3 UCs had allelic loss at this region. RT-PCR analysis of the LRP1B gene showed the lack of expression of several exons in 2 of 9 cases analyzed. Our analysis suggests that the LRP1B gene is a candidate tumor suppressor gene in UCs.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carcinoma / classification
  • Carcinoma / genetics*
  • Carcinoma / pathology
  • Cell Cycle Proteins / genetics
  • Cell Cycle Proteins / metabolism
  • Chromosomes, Human, Pair 2
  • Cullin Proteins*
  • DNA, Neoplasm / analysis
  • Humans
  • Loss of Heterozygosity*
  • Male
  • Microsatellite Repeats
  • Nucleic Acid Hybridization
  • Receptors, LDL / genetics*
  • Receptors, LDL / metabolism
  • Reverse Transcriptase Polymerase Chain Reaction
  • Urinary Bladder Neoplasms / classification
  • Urinary Bladder Neoplasms / genetics*
  • Urinary Bladder Neoplasms / pathology
  • Urothelium / pathology

Substances

  • CUL3 protein, human
  • Cell Cycle Proteins
  • Cullin Proteins
  • DNA, Neoplasm
  • LRP1B protein, human
  • Receptors, LDL